A new micro swim-up procedure for sperm preparation in ICSI treatments: preliminary microbiological testing
نویسندگان
چکیده
OBJECTIVE This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure. METHODS Semen analysis and microbiological tests were carried out before and after the MSU procedure. A total of twenty semen samples were analyzed. RESULTS Pathogens were observed in semen samples only before MSU and never after ICSI. Microbiological tests revealed a large prevalence of gram-positive cocci [Staphylococcus spp. (n=16, 80%) and viridans streptococci (n=10, 50%)]. The results of this study indicate that direct MSU in ICSI improved the ICSI workflow. CONCLUSION The new workflow is faster and more affordable, and is likely to prevent infection problems that could arise from the normal microbial flora of the semen.
منابع مشابه
Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
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